GDNF or Glial Derived Neurotrophic factor Human ELISA (Enzyme Linked Immunosorbent Assay Kit) is an in-vitro enzyme link immunosorbent assay that measures the quantity of Human GDNF found in plasma and cells culture supernatants. This assay may not detect the human GDNF concentration in plasma.
The assay uses an antibody that is specific for Human GDNF and coated on a 96 well plate. The immobilized antibody is applied to the wells of the plates and the GDNF in the samples and standards are pipetted into them. After washing the wells, biotinylated anti Human GDNF antibody is added. You can buy top GDNF ELISA kit via Boster Bio.
The amount of GDNF bound to the color will affect the development of the color. The Stop Solution changes the color to yellow and measures the intensity at 450 nm. Only serum spiked with Human GDNF was able to detect the endogenous Human GDNF. Glial cell-line derived neurotrophic factors (GDNF), is a highly conserved member of the TGF Beta superfamily.
The GDNF gene product can be processed into a disulphide linked homodimer that is a ligand to the RET proto oncogene. GDNF repairs and protects dopamine-containing neuronal cells that are degenerating in Parkinson's disease and motor neurons that die in amyotrophic lateral sclerosis. In the clinic, GDNF has been shown to be effective in treating Parkinson's disease.
Neurological improvement has also been seen in the treatment of spinal cord injuries using GDNF. Human Glial Derived Neurotrophic Factor Hu or GDNF ELISA quantifies Hu GDNF levels in serum, plasma and cell culture media. This assay can only recognize natural and recombinant Hu GDNF. The microplate has wells that have been pre-coated with the target-specific antibody.